SINGLE-MOLECULE FLUORESCENCE SPECTROSCOPY

  Confocal fluorescence image where bright spots are single fluorescent molecules bound to DNA duplexes.       

Confocal fluorescence image where bright spots are single fluorescent molecules bound to DNA duplexes.

 

 

Single-molecule spectroscopy goes beyond ensemble-averaged information to uncover asynchronous behavior and heterogeneity, characteristics intrinsic to biological systems. We employ and develop single-molecule methods to explore dynamics. Single-molecule Förster resonance energy transfer (smFRET) enables the study of important structural dynamics under physiological conditions. These single-molecule experiments allow us to investigate a wide variety of systems ranging from biological receptors to natural and artificial light harvesting systems. Our lab is also developing an ultrafast single-molecule experiment to study heterogeneity in energy transfer dynamics in photosynthetic systems.

T. Kondo, W. J. Chen, G. S. Schlau-Cohen, "Single-Molecule Fluorescence Spectroscopy of Photosynthetic Systems." Chem Rev117, 860 (2017).

Group Members: Toru, Julianne, Raju, Crystal, Jesse, Shwetha, Ray